Journal: Frontiers in Pharmacology
Article Title: Evaluation of in vitro pharmacological activities of medicinal mushrooms in the context of dry eye disease
doi: 10.3389/fphar.2025.1557359
Figure Lengend Snippet: Effects of fungal extracts on the TEER (A, B, C) and fluorescein permeability (D) of HCE-T cells. (A, B, C) HCE-T cells were cultured on a translucent membrane of a transwell insert for 72 h and treated with mushroom extracts. The TEER was measured every 24 h. (D) After TEER measurement, cells were washed and exposed to fluorescein for 2 h to determine permeability. BAC was used as a positive control. Data were normalised to 4% FCS and presented as mean ± standard deviation. n = 3; *p < 0.05, **p < 0.01, ***p < 0.001.
Article Snippet: HCE-T cells (3 × 10 4 cells) were inoculated with 400 μL of culture medium onto a translucent polyester (PET) membrane transwell insert (0.4 μm pore size; Greiner bio-one), and 1 mL of culture medium was added into the basolateral chamber.
Techniques: Permeability, Cell Culture, Membrane, Positive Control, Standard Deviation